نشریه پژوهش هایی در کشت سلول و بافت کاریوتیک
سال یکم شماره 1 (تابستان 1399)

Echis carinatus are small in size and are considered to be very biting invasive snakes. Snake venom is a type of snake saliva that is secreted through the salivary glands and stored in the venom sac. A problem in human societies, especially in rural areas, is snake bites, which are not treated properly. The aim of this study is evaluation of toxicity of saliva of Echis carinatus sochureki on cells growth and the mechanisms involved.

In this study, the effect of Echis carinatus sochureki saliva on human dermal fibroblasts (HDF) cells growth was determined by the inverted microscope, MTT assay, and Neutral red assay. The integrity of the cell membrane through LDH release was also measured.

The MTT assay and Neutral red assay showed a significant (p < /em>˂0.001) cytotoxic effect of Echis carinatus sochureki salvia on HDF cells growth after 24 hours treatment. Also, Echis carinatus sochureki venom caused a significant (p < /em>˂0.001) increase in LDH release. Various morphological abnormalities were observed in cells.

The Echis carinatus sochureki saliva causes cytotoxic effects on HDF cells by the necrotic mechanism. The results of this study will be useful for future research in this field, as well as therapeutic methods for snake bites.

According to the WHO definition, recurrent miscarriage is a consecutive pregnancy loss before weeks 22. Different factors involved in recurrent abortion as a multifactorial disease. Genetic factors are one of the important factors associated with recurrent abortion. VEGFA (Vascular Endothelial Growth Factor A) is one of the important gene in angiogenesis. MTHFD1 (Methylenetetrahydrofolate Dehydrogenase, Cyclohydrolase, And Formyltetrahydrofolate Synthetase 1) is a protein-coding gene that metabolism folate. The goal of this article is to assess the association between single nucleotide polymorphisms of VEGFA and MTHFD1 and the recurrent miscarriage in Iranian women.

The study group was 50 women with recurrent miscarriages and the control groups consisted of 10 women with at least two successful pregnancies and no miscarriages. The DNA extracted from leucocytes cells. PCR was used to find the association between VEGFA and MTHFD1 genes and recurrent miscarriages. Analyzing performed with FinchTV software after data sequencing.

Our results showed that the polymorphism frequency of CC and CT in rs2236225 VEGFA were 86% and 14% in women with recurrent miscarriages and 90% and 10% in the control group. the polymorphism frequency of GG and GA in rs3025039MTHFD1 were 82% and 18% in women with recurrent miscarriages and no polymorphism was detected in the control group.

These results showed that there is an association between single nucleotide polymorphisms of VEGFA and MTHFD1 and the recurrent miscarriage in Iranian women. Furthermore, MTHFD1 polymorphism could be a molecular marker in the diagnosis of recurrent miscarriage.

Opium addiction is one of the most prevalent addiction in Iranian society. Throughout the last two decades, congress 60; a nongovernmental organization; has been operated a taper off treatment of opium associated with a package of psychological treatment group classes. Although the effectiveness of the taper off method in opium addiction has been verified, molecular mechanisms involved in this treatment have not explained. BDNF gene is a brain-derived neurotrophic factor that is associated with numerous molecular mechanisms of the brain including memory.

In this research, peripheral blood samples from 21 patients were collected from Congress 60. RNA was extracted from each sample, reverse transcribed and amplified via RT-PCR technique, utilizing specific primers for BDNF. The production of BDNF protein was also analyzed employing the western blotting technique.

Our results exhibited considerable down expression of BDNF in addict persons vs. non-psychiatric persons (p < /em><0.01). Furthermore, BDNF expression levels in addicts increased significantly after the therapy period. Findings showed the effect of opium abuse and taper off treatment on the expression of BDNF.

The present study could help to a better understanding of molecular and neurological mechanisms of opium and taper off treatment. Additionally, BDNF level detection could be a potential marker for screening the effectiveness of various types of addiction treatment.

Breast cancer is the most common cancer among Iranian women and women in the world. The aim of radiotherapy (radiation therapy) is to eliminate the maximum of cancer cells with minimal damage to healthy tissue. Intraoperative radiation therapy (IORT) can be utilized to treat a small area of tissue without any damages. In this method, because of DNA damage and initiation of the apoptosis process, the tumor cells (target tissue) are destructed.

Four Breast Cancer (BC) patients as a pilot study were treated by 21 Gy (Radical dose). Two-dimensional electrophoresis (2D-PAGE) and Progenesis Same Spots v3.2 were performed to study the proteomic of the IORT-treated tumor bed.

Approximately 523 protein spots were detected. A total of 343 protein spots showed different expression. among which, 141 spots decrease in-expression level, while, the remaining 191 spots were up-regulated.

Our findings indicate that Caspase3, P53, BID, BAX, and BCL2 may be key proteins in the IORT-treated tumor bed.

Cyanobacteria, microscopic organisms have similar characteristics to prokaryotes and eukaryotes, which are very popular in biotechnology research today and are able to produce a wide range of highly valuable organic compounds. However, many strains of cyanobacteria still remain unknown in Iran, a group that is dedicated to freshwater (ponds or springs and streams) and a group that is related to terrestrial habitats. This is in many cases due to the fact that the specific cultivation methods of cyanobacterial strains are not well known. In fact, culturing the strains of axenic cyanobacteria, free of any bacteria or other pathogens, will allow many research activities to be performed in various genetic and molecular fields. In this article, in order to get acquainted with different media cultures for different types of cyanobacterial strains, the correct and accurate method of isolation, cultivation and purification of aquatic and terrestrial, cyanobacteria will be explained.

The cell cultures, especially primary cultures, are widely used in the isolation and identification of viruses. Viruses are one of the most important causes of disease and can cause enormous economic damage. Also, they are used in basic research in cell physiology, genetics, and many other items. Primary cell cultures are also used in chromosomal research on karyotypes, morphology, and abnormal states of chromosomes. Primary cell culture preparation, such as bees that have not yet been derived from the cell line, is widely used in the isolation and identification of viruses. In the field of honey bee cell culture, the research in most countries is more limited than other insects but in recent years, good results have been achieved in this field. There are some good results about the larval and pupal stages of the honey bee primary culture in Iran. Since there are some reports about the influence of honey bee in viral investigation in Iran, this article introduces methods of bee cell culture preparation and the results obtained in Iran and other countries that can use to isolate viruses and study them when necessary.